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pe conjugated mouse anti chicken cd8  (Bio-Rad)


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    Bio-Rad pe conjugated mouse anti chicken cd8
    Pe Conjugated Mouse Anti Chicken Cd8, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+conjugated+mouse+anti+chicken+cd8/Mouse+anti+Chicken+CD8+Alpha/pmc12796584-164-29-34
    Average 91 stars, based on 13 article reviews
    pe conjugated mouse anti chicken cd8 - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    FACS:

    Article Title: Effects of incompletely degraded galactomannan on growth performance and immune function of cyclophosphamide-induced immunosuppressed broilers
    Article Snippet: .. An aliquot of 100 μL (containing 1 × 10 6 cells) was transferred into a FACS tube, incubated with FITC-conjugated Mouse anti-Chicken CD4 (#MCA2164F, Bio-Rad, Hercules, CA, USA) and PE-conjugated Mouse anti-Chicken CD8 (#MCA2166PE, Bio-Rad) antibodies at 4°C for 30 min. ..

    Article Title: Effects of incompletely degraded galactomannan on growth performance and immune function of cyclophosphamide-induced immunosuppressed broilers.
    Article Snippet: .. An aliquot of 100 μL (containing 1 × 106 cells) was transferred into a FACS tube, incubated with FITCconjugated Mouse anti-Chicken CD4 (#MCA2164F, Bio-Rad, Hercules, CA, USA) and PE-conjugated Mouse anti-Chicken CD8 (#MCA2166PE, Bio-Rad) antibodies at 4◦C for 30 min. ..

    Incubation:

    Article Title: Effects of incompletely degraded galactomannan on growth performance and immune function of cyclophosphamide-induced immunosuppressed broilers
    Article Snippet: .. An aliquot of 100 μL (containing 1 × 10 6 cells) was transferred into a FACS tube, incubated with FITC-conjugated Mouse anti-Chicken CD4 (#MCA2164F, Bio-Rad, Hercules, CA, USA) and PE-conjugated Mouse anti-Chicken CD8 (#MCA2166PE, Bio-Rad) antibodies at 4°C for 30 min. ..

    Article Title: Effects of incompletely degraded galactomannan on growth performance and immune function of cyclophosphamide-induced immunosuppressed broilers.
    Article Snippet: .. An aliquot of 100 μL (containing 1 × 106 cells) was transferred into a FACS tube, incubated with FITCconjugated Mouse anti-Chicken CD4 (#MCA2164F, Bio-Rad, Hercules, CA, USA) and PE-conjugated Mouse anti-Chicken CD8 (#MCA2166PE, Bio-Rad) antibodies at 4◦C for 30 min. ..



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    Effect of arginine supplementation on the ratio of <t>CD8</t> + to CD4 + T cells in the spleen of broilers during NE challenge. On d 14, the birds in the NE + Basal, NE + Arg 125%, and NE + Arg 135% groups were orally gavaged with 1 × 10 4 sporulated oocysts of E. maxima and on d 19, 20, and 21, the birds were gavaged with 1 × 10 8 CFU/mL of C. perfringens . The spleen was collected on d 21, 28, and 35, and flow cytometry was performed to evaluate the effect of arginine supplementation on the CD8 + : CD4 + ratio during the NE challenge. Bars (± SEM) with no common superscript differ significantly ( P < 0.05). P -values for D21: P = 0.26, D28: P = 0.01, D35: P < 0.01.
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    Thymus and bursa of Fabricius sections showing (a) isotype control. (b) Bursa of Fabricius cell showing lymphocytes expressing Bu-1 marker (brown in color). (c) Thymocytes expressing CD4 marker (brown-stained cells). (d) Thymocytes expressing <t>CD8</t> marker (brown-stained cells). Primary antibody and biotinylated horse anti-mouse <t>IgG</t> secondary antibody were the first to treat the section. A ”charged” DAB (Abcam, USA) was the primary stain to develop color and methyl green was the counterstain.
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    Effect of arginine supplementation on the ratio of CD8 + to CD4 + T cells in the spleen of broilers during NE challenge. On d 14, the birds in the NE + Basal, NE + Arg 125%, and NE + Arg 135% groups were orally gavaged with 1 × 10 4 sporulated oocysts of E. maxima and on d 19, 20, and 21, the birds were gavaged with 1 × 10 8 CFU/mL of C. perfringens . The spleen was collected on d 21, 28, and 35, and flow cytometry was performed to evaluate the effect of arginine supplementation on the CD8 + : CD4 + ratio during the NE challenge. Bars (± SEM) with no common superscript differ significantly ( P < 0.05). P -values for D21: P = 0.26, D28: P = 0.01, D35: P < 0.01.

    Journal: Poultry Science

    Article Title: Effect of 125% and 135% arginine on the growth performance, intestinal health, and immune responses of broilers during necrotic enteritis challenge

    doi: 10.1016/j.psj.2024.103826

    Figure Lengend Snippet: Effect of arginine supplementation on the ratio of CD8 + to CD4 + T cells in the spleen of broilers during NE challenge. On d 14, the birds in the NE + Basal, NE + Arg 125%, and NE + Arg 135% groups were orally gavaged with 1 × 10 4 sporulated oocysts of E. maxima and on d 19, 20, and 21, the birds were gavaged with 1 × 10 8 CFU/mL of C. perfringens . The spleen was collected on d 21, 28, and 35, and flow cytometry was performed to evaluate the effect of arginine supplementation on the CD8 + : CD4 + ratio during the NE challenge. Bars (± SEM) with no common superscript differ significantly ( P < 0.05). P -values for D21: P = 0.26, D28: P = 0.01, D35: P < 0.01.

    Article Snippet: Single-cell suspensions of cecal tonsil and spleen lymphocytes were obtained and incubated with FITC-conjugated mouse anti-chicken CD4 at 1:250 dilution, PE-conjugated mouse anti-chicken CD8 (Southern Biotech, Birmingham, AL) at 1:450 dilution, and unlabeled mouse IgG at 1:100 dilution in a 96 well plate and incubated for 20 min. Also, single-cell suspensions of cecal tonsil and spleen lymphocytes were incubated with PE-conjugated mouse anti-chicken CD25 (Southern Biotech, Birmingham, AL) at 1:10 dilution and unlabeled mouse IgG at 1:100 dilution in a 96 well plate and incubated for 40 minutes.

    Techniques: Flow Cytometry

    Effect of arginine supplementation on the ratio of CD8 + to CD4 + T cells in the cecal tonsil of broilers during NE challenge. On d 14, the birds in the NE + Basal, NE + Arg 125%, and NE + Arg 135% groups were orally gavaged with 1 × 10 4 sporulated oocysts of E. maxima and on d 19, 20, and 21, the birds were gavaged with 1 × 10 8 CFU/mL of C. perfringens . Cecal tonsils were collected on d 21, 28, and 35, and flow cytometry was performed to evaluate the effect of arginine supplementation on the CD8 + : CD4 + ratio during the NE challenge. Bars (± SEM) with no common superscript differ significantly ( P < 0.05). P -values for D21: P = 0.05, D28: P = 0.09, D35: P = 0.03.

    Journal: Poultry Science

    Article Title: Effect of 125% and 135% arginine on the growth performance, intestinal health, and immune responses of broilers during necrotic enteritis challenge

    doi: 10.1016/j.psj.2024.103826

    Figure Lengend Snippet: Effect of arginine supplementation on the ratio of CD8 + to CD4 + T cells in the cecal tonsil of broilers during NE challenge. On d 14, the birds in the NE + Basal, NE + Arg 125%, and NE + Arg 135% groups were orally gavaged with 1 × 10 4 sporulated oocysts of E. maxima and on d 19, 20, and 21, the birds were gavaged with 1 × 10 8 CFU/mL of C. perfringens . Cecal tonsils were collected on d 21, 28, and 35, and flow cytometry was performed to evaluate the effect of arginine supplementation on the CD8 + : CD4 + ratio during the NE challenge. Bars (± SEM) with no common superscript differ significantly ( P < 0.05). P -values for D21: P = 0.05, D28: P = 0.09, D35: P = 0.03.

    Article Snippet: Single-cell suspensions of cecal tonsil and spleen lymphocytes were obtained and incubated with FITC-conjugated mouse anti-chicken CD4 at 1:250 dilution, PE-conjugated mouse anti-chicken CD8 (Southern Biotech, Birmingham, AL) at 1:450 dilution, and unlabeled mouse IgG at 1:100 dilution in a 96 well plate and incubated for 20 min. Also, single-cell suspensions of cecal tonsil and spleen lymphocytes were incubated with PE-conjugated mouse anti-chicken CD25 (Southern Biotech, Birmingham, AL) at 1:10 dilution and unlabeled mouse IgG at 1:100 dilution in a 96 well plate and incubated for 40 minutes.

    Techniques: Flow Cytometry

    Thymus and bursa of Fabricius sections showing (a) isotype control. (b) Bursa of Fabricius cell showing lymphocytes expressing Bu-1 marker (brown in color). (c) Thymocytes expressing CD4 marker (brown-stained cells). (d) Thymocytes expressing CD8 marker (brown-stained cells). Primary antibody and biotinylated horse anti-mouse IgG secondary antibody were the first to treat the section. A ”charged” DAB (Abcam, USA) was the primary stain to develop color and methyl green was the counterstain.

    Journal: Veterinary World

    Article Title: Development of lymphocyte subpopulations in local breed chickens

    doi: 10.14202/vetworld.2021.1846-1852

    Figure Lengend Snippet: Thymus and bursa of Fabricius sections showing (a) isotype control. (b) Bursa of Fabricius cell showing lymphocytes expressing Bu-1 marker (brown in color). (c) Thymocytes expressing CD4 marker (brown-stained cells). (d) Thymocytes expressing CD8 marker (brown-stained cells). Primary antibody and biotinylated horse anti-mouse IgG secondary antibody were the first to treat the section. A ”charged” DAB (Abcam, USA) was the primary stain to develop color and methyl green was the counterstain.

    Article Snippet: Alternatively, mouse anti-chicken CD4-FITC-conjugated mAb (mouse IgG1) (Southern Biotech) and mouse anti-chicken CD8-PE-conjugated mAb (mouse IgG1) (Southern Biotech) were used in the two-color, direct immunofluorescent staining procedure to identify the CD4 + and/or CD8 + markers, respectively, on the T lymphocytes.

    Techniques: Control, Expressing, Marker, Staining

    Spleen section showing: (a) Isotype control. (b) CD8 (CD4 – CD8 + ) lymphocyte stained in brown color especially in red bulb (RP) 40×. (c) Lymphocytes expressing CD3 marker 100×. (d) Lymphocytes expressing Bu-1 marker 100×. Primary antibody and biotinylated horse anti-mouse IgG secondary antibody were the first to treat the section. A ”charged” DAB (Abcam, USA) was the primary stain to develop color and methyl green was the counterstain.

    Journal: Veterinary World

    Article Title: Development of lymphocyte subpopulations in local breed chickens

    doi: 10.14202/vetworld.2021.1846-1852

    Figure Lengend Snippet: Spleen section showing: (a) Isotype control. (b) CD8 (CD4 – CD8 + ) lymphocyte stained in brown color especially in red bulb (RP) 40×. (c) Lymphocytes expressing CD3 marker 100×. (d) Lymphocytes expressing Bu-1 marker 100×. Primary antibody and biotinylated horse anti-mouse IgG secondary antibody were the first to treat the section. A ”charged” DAB (Abcam, USA) was the primary stain to develop color and methyl green was the counterstain.

    Article Snippet: Alternatively, mouse anti-chicken CD4-FITC-conjugated mAb (mouse IgG1) (Southern Biotech) and mouse anti-chicken CD8-PE-conjugated mAb (mouse IgG1) (Southern Biotech) were used in the two-color, direct immunofluorescent staining procedure to identify the CD4 + and/or CD8 + markers, respectively, on the T lymphocytes.

    Techniques: Control, Staining, Expressing, Marker

    Flow cytometry results of cell suspension from spleen, thymus, and bursa of Fabricius. T lymphocytes (CD8 in particular with CD4 – CD8 + marker) were the dominant cells in the spleen. Thymocytes show that the CD3 marker is denser than that in bursa. Conversely, the Bu-1 marker is highly expressed by the lymphocytes in bursa. All the lymphocytes in the thymus were expressing both CD4 and CD8 markers (CD4 + CD8 + ) with traces of CD4 – CD8 - , CD4 + CD8 – , and CD4 – CD8 + . In the bursa, almost all the lymphocytes are expressing no marker (CD4 – CD8 + ).

    Journal: Veterinary World

    Article Title: Development of lymphocyte subpopulations in local breed chickens

    doi: 10.14202/vetworld.2021.1846-1852

    Figure Lengend Snippet: Flow cytometry results of cell suspension from spleen, thymus, and bursa of Fabricius. T lymphocytes (CD8 in particular with CD4 – CD8 + marker) were the dominant cells in the spleen. Thymocytes show that the CD3 marker is denser than that in bursa. Conversely, the Bu-1 marker is highly expressed by the lymphocytes in bursa. All the lymphocytes in the thymus were expressing both CD4 and CD8 markers (CD4 + CD8 + ) with traces of CD4 – CD8 - , CD4 + CD8 – , and CD4 – CD8 + . In the bursa, almost all the lymphocytes are expressing no marker (CD4 – CD8 + ).

    Article Snippet: Alternatively, mouse anti-chicken CD4-FITC-conjugated mAb (mouse IgG1) (Southern Biotech) and mouse anti-chicken CD8-PE-conjugated mAb (mouse IgG1) (Southern Biotech) were used in the two-color, direct immunofluorescent staining procedure to identify the CD4 + and/or CD8 + markers, respectively, on the T lymphocytes.

    Techniques: Flow Cytometry, Suspension, Marker, Expressing

    Determination of lymphocyte population subsets in lymphoid organs.

    Journal: Veterinary World

    Article Title: Development of lymphocyte subpopulations in local breed chickens

    doi: 10.14202/vetworld.2021.1846-1852

    Figure Lengend Snippet: Determination of lymphocyte population subsets in lymphoid organs.

    Article Snippet: Alternatively, mouse anti-chicken CD4-FITC-conjugated mAb (mouse IgG1) (Southern Biotech) and mouse anti-chicken CD8-PE-conjugated mAb (mouse IgG1) (Southern Biotech) were used in the two-color, direct immunofluorescent staining procedure to identify the CD4 + and/or CD8 + markers, respectively, on the T lymphocytes.

    Techniques: