pe conjugated mouse anti chicken cd8  (Bio-Rad)

Journal: Poultry Science

Article Title: Effect of 125% and 135% arginine on the growth performance, intestinal health, and immune responses of broilers during necrotic enteritis challenge

doi: 10.1016/j.psj.2024.103826

Figure Lengend Snippet: Effect of arginine supplementation on the ratio of CD8 + to CD4 + T cells in the spleen of broilers during NE challenge. On d 14, the birds in the NE + Basal, NE + Arg 125%, and NE + Arg 135% groups were orally gavaged with 1 × 10 4 sporulated oocysts of E. maxima and on d 19, 20, and 21, the birds were gavaged with 1 × 10 8 CFU/mL of C. perfringens . The spleen was collected on d 21, 28, and 35, and flow cytometry was performed to evaluate the effect of arginine supplementation on the CD8 + : CD4 + ratio during the NE challenge. Bars (± SEM) with no common superscript differ significantly ( P < 0.05). P -values for D21: P = 0.26, D28: P = 0.01, D35: P < 0.01.

Article Snippet: Single-cell suspensions of cecal tonsil and spleen lymphocytes were obtained and incubated with FITC-conjugated mouse anti-chicken CD4 at 1:250 dilution, PE-conjugated mouse anti-chicken CD8 (Southern Biotech, Birmingham, AL) at 1:450 dilution, and unlabeled mouse IgG at 1:100 dilution in a 96 well plate and incubated for 20 min. Also, single-cell suspensions of cecal tonsil and spleen lymphocytes were incubated with PE-conjugated mouse anti-chicken CD25 (Southern Biotech, Birmingham, AL) at 1:10 dilution and unlabeled mouse IgG at 1:100 dilution in a 96 well plate and incubated for 40 minutes.

Techniques: Flow Cytometry

Journal: Poultry Science

Article Title: Effect of 125% and 135% arginine on the growth performance, intestinal health, and immune responses of broilers during necrotic enteritis challenge

doi: 10.1016/j.psj.2024.103826

Figure Lengend Snippet: Effect of arginine supplementation on the ratio of CD8 + to CD4 + T cells in the cecal tonsil of broilers during NE challenge. On d 14, the birds in the NE + Basal, NE + Arg 125%, and NE + Arg 135% groups were orally gavaged with 1 × 10 4 sporulated oocysts of E. maxima and on d 19, 20, and 21, the birds were gavaged with 1 × 10 8 CFU/mL of C. perfringens . Cecal tonsils were collected on d 21, 28, and 35, and flow cytometry was performed to evaluate the effect of arginine supplementation on the CD8 + : CD4 + ratio during the NE challenge. Bars (± SEM) with no common superscript differ significantly ( P < 0.05). P -values for D21: P = 0.05, D28: P = 0.09, D35: P = 0.03.

Article Snippet: Single-cell suspensions of cecal tonsil and spleen lymphocytes were obtained and incubated with FITC-conjugated mouse anti-chicken CD4 at 1:250 dilution, PE-conjugated mouse anti-chicken CD8 (Southern Biotech, Birmingham, AL) at 1:450 dilution, and unlabeled mouse IgG at 1:100 dilution in a 96 well plate and incubated for 20 min. Also, single-cell suspensions of cecal tonsil and spleen lymphocytes were incubated with PE-conjugated mouse anti-chicken CD25 (Southern Biotech, Birmingham, AL) at 1:10 dilution and unlabeled mouse IgG at 1:100 dilution in a 96 well plate and incubated for 40 minutes.

Techniques: Flow Cytometry

Journal: Veterinary World

Article Title: Development of lymphocyte subpopulations in local breed chickens

doi: 10.14202/vetworld.2021.1846-1852

Figure Lengend Snippet: Thymus and bursa of Fabricius sections showing (a) isotype control. (b) Bursa of Fabricius cell showing lymphocytes expressing Bu-1 marker (brown in color). (c) Thymocytes expressing CD4 marker (brown-stained cells). (d) Thymocytes expressing CD8 marker (brown-stained cells). Primary antibody and biotinylated horse anti-mouse IgG secondary antibody were the first to treat the section. A ”charged” DAB (Abcam, USA) was the primary stain to develop color and methyl green was the counterstain.

Article Snippet: Alternatively, mouse anti-chicken CD4-FITC-conjugated mAb (mouse IgG1) (Southern Biotech) and mouse anti-chicken CD8-PE-conjugated mAb (mouse IgG1) (Southern Biotech) were used in the two-color, direct immunofluorescent staining procedure to identify the CD4 + and/or CD8 + markers, respectively, on the T lymphocytes.

Techniques: Control, Expressing, Marker, Staining

Journal: Veterinary World

Article Title: Development of lymphocyte subpopulations in local breed chickens

doi: 10.14202/vetworld.2021.1846-1852

Figure Lengend Snippet: Spleen section showing: (a) Isotype control. (b) CD8 (CD4 – CD8 + ) lymphocyte stained in brown color especially in red bulb (RP) 40×. (c) Lymphocytes expressing CD3 marker 100×. (d) Lymphocytes expressing Bu-1 marker 100×. Primary antibody and biotinylated horse anti-mouse IgG secondary antibody were the first to treat the section. A ”charged” DAB (Abcam, USA) was the primary stain to develop color and methyl green was the counterstain.

Article Snippet: Alternatively, mouse anti-chicken CD4-FITC-conjugated mAb (mouse IgG1) (Southern Biotech) and mouse anti-chicken CD8-PE-conjugated mAb (mouse IgG1) (Southern Biotech) were used in the two-color, direct immunofluorescent staining procedure to identify the CD4 + and/or CD8 + markers, respectively, on the T lymphocytes.

Techniques: Control, Staining, Expressing, Marker

Journal: Veterinary World

Article Title: Development of lymphocyte subpopulations in local breed chickens

doi: 10.14202/vetworld.2021.1846-1852

Figure Lengend Snippet: Flow cytometry results of cell suspension from spleen, thymus, and bursa of Fabricius. T lymphocytes (CD8 in particular with CD4 – CD8 + marker) were the dominant cells in the spleen. Thymocytes show that the CD3 marker is denser than that in bursa. Conversely, the Bu-1 marker is highly expressed by the lymphocytes in bursa. All the lymphocytes in the thymus were expressing both CD4 and CD8 markers (CD4 + CD8 + ) with traces of CD4 – CD8 - , CD4 + CD8 – , and CD4 – CD8 + . In the bursa, almost all the lymphocytes are expressing no marker (CD4 – CD8 + ).

Article Snippet: Alternatively, mouse anti-chicken CD4-FITC-conjugated mAb (mouse IgG1) (Southern Biotech) and mouse anti-chicken CD8-PE-conjugated mAb (mouse IgG1) (Southern Biotech) were used in the two-color, direct immunofluorescent staining procedure to identify the CD4 + and/or CD8 + markers, respectively, on the T lymphocytes.

Techniques: Flow Cytometry, Suspension, Marker, Expressing

Journal: Veterinary World

Article Title: Development of lymphocyte subpopulations in local breed chickens

doi: 10.14202/vetworld.2021.1846-1852

Figure Lengend Snippet: Determination of lymphocyte population subsets in lymphoid organs.

Article Snippet: Alternatively, mouse anti-chicken CD4-FITC-conjugated mAb (mouse IgG1) (Southern Biotech) and mouse anti-chicken CD8-PE-conjugated mAb (mouse IgG1) (Southern Biotech) were used in the two-color, direct immunofluorescent staining procedure to identify the CD4 + and/or CD8 + markers, respectively, on the T lymphocytes.

Techniques:

Journal: Parasites & Vectors

Article Title: Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene

doi: 10.1186/s13071-018-2906-5

Figure Lengend Snippet: T lymphocytes subpopulations were detected by the flow cytometry technique. a CD4 + T lymphocytes (CD3 + CD4 + , region Q2). b CD8 + T lymphocytes (CD3 + CD8 + , region Q2)

Article Snippet: The cells were then probed with PE-conjugated mouse monoclonal anti-chicken CD4 or the PE-conjugated mouse monoclonal anti-chicken CD8 (Southern Biotechnology Associates, Birmingham, AL, USA).

Techniques: Flow Cytometry

Journal: Parasites & Vectors

Article Title: Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene

doi: 10.1186/s13071-018-2906-5

Figure Lengend Snippet: Flow cytometry analysis of the percentages of T lymphocyte subsets (mean ± SD, %)

Article Snippet: The cells were then probed with PE-conjugated mouse monoclonal anti-chicken CD4 or the PE-conjugated mouse monoclonal anti-chicken CD8 (Southern Biotechnology Associates, Birmingham, AL, USA).

Techniques: Flow Cytometry, Marker, Control